Isolation and characterization of a partial fragment (tRNA-Lys' to tRNA-Leu') of the mitochondrial genome of Rasbora sarawakensis

Chew, Pei Ling (2017) Isolation and characterization of a partial fragment (tRNA-Lys' to tRNA-Leu') of the mitochondrial genome of Rasbora sarawakensis. [Final Year Project Report] (Unpublished)

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Abstract

Mitochondrial genome is used in phylogenetic studies because of its fast evolutionary rate, lack of genetic recombination and compact gene organization. As there are lack of phylogenetic relationship study in the genus rasbora, mitochondrial DNA can be used to construct a phylogenetic network that showed the evolution relationship between the species. The aim of this study is to isolate and characterize the partial fragment of mitochondrial genome of Rasbora sarawakensis. The total DNA was extracted and primer walking approach was applied to isolate the partial fragment (tRNA-Lys' to tRNA-Leu) with expected size of 4000 bp. By designing five set of primer from conserved sequences from the multiple alignment of three species which are Rasbora sumatrana, Rasbora trilineata and Rasbora steneri. The PCR amplification was successfully performed for all the five sequences and subsequently these fragments were assembled using MEGA7 software into a single consensus sequence yielding 4016 bp. The sequence was also blasted in order to further verify its identity with other species from the genbank. Through phylogenetic analysis performed, it was found that Rasbora sarawakensis had 67.9% of bootstraps value with R. vaterihoris. Hence, it is suggested that overall mitochondrial genome can be used to better represent a more in-depth and reliable approach in understanding the phylogenetic relationship in Rasbora spp. than morphological approach in its identification.

Item Type: Final Year Project Report
Additional Information: Project Report (BSc.) - Universiti Malaysia Sarawak, 2017.
Uncontrolled Keywords: Mitochondrial genome, Phylogenetic analysis, Rasbora Sarawakensis, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 06 Nov 2019 03:47
Last Modified: 10 Sep 2021 13:01
URI: http://ir.unimas.my/id/eprint/27712

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