PCR Optimization and PCR-RFLP Analysis of Ambal Biasa (Solen regularis) from Kuching Bay Areas

Crocker, Melinda Alexandria (2007) PCR Optimization and PCR-RFLP Analysis of Ambal Biasa (Solen regularis) from Kuching Bay Areas. [Final Year Project Report] (Unpublished)

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Abstract

This study was conducted to obtain a baseline data for molecular studies of 'ambal biasa' (Solen regularis) in Kuching Bay area. Samples were collected from tlu·ee sites namely Bako, Asajaya and Buntal in the month of September 2006. There were 38 individuals of S. regularis that were used in this study. The modified CT AB protocol was used in utilizing the total genomic DNA ofthe S. regularis. PCR had been carried out to amplify the l6S mtDNA gene and ITS··1 gene region. Primers 16Sar-L (Forward) and 16Sbr-H (Reverse) were used for l6S mtDNA while ETTS-2 (Forward) and ITS-l r (Reverse) were used for ITS-l gene. The PCR annealing temperature acquired for gene 16S mtDNA was at 45°Celsius whereas the optimal temperature was at 54°Celsius for ITS-l gene. Four enzymes had been used in PCRRFLP experiment namely Alu1, BamH1, Mps1 , Sau3A and BSlz1. However, only RE Alul showed positive result.

Item Type: Final Year Project Report
Additional Information: Project Report (BSc.) - Universiti Malaysia Sarawak, 2007.
Uncontrolled Keywords: Solen regularis, PCR-RFLP analyses, annealing temperature, unimas, university, universiti, Borneo, Malaysia. Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 06 Nov 2019 00:55
Last Modified: 06 Nov 2019 00:55
URI: http://ir.unimas.my/id/eprint/27683

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