Transformation of mungbean cotyledon by using agrobacterium tumefaciens

Syafiqah Diana, Binti Subandi. (2015) Transformation of mungbean cotyledon by using agrobacterium tumefaciens. [Final Year Project Report] (Unpublished)

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Mungbean (Vigna radiata (Ll Wilezek) was transformed by using Agroboclerium lume(oeiel1s strain LBA 4404. Plasmid pGPTV-SRN4/ALS was cloned into the Agrobacleriul17 lume(aeiens . . Two types of explants were used in this researches which are the leaf cotyledon and cotyledon attached with embryonalax is (CAEA). These explants were co-culti va ted with Agrobaelerium lume(ociens in th e presence of acetosyringone. Binary vector harbouring luei/erase (LUe:') gene (pGPTV), was tested to examine its effec t on transformation efficiency. The cotyledons from treated and germinated seedlings anal ysed via polymerase chain reaction (peR). The res ult obtai ned fro m the comparison of the efficiency of cotyledon leaf (2-3 stages leaves) and cotyledon attached with embryonal axis were inconclusive . For further studies, the transformation efficiency needs 'to be in creased and also improve on the genomic DNA ex trac tion procedure.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2015.
Uncontrolled Keywords: Mungbean, A. tumefaciens, explants transformation, cotyledons, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 14 Oct 2019 04:43
Last Modified: 14 Oct 2019 04:43

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