Quantitative and comparative analysis of WT1 (Wilms tumour suppressor) gene expression in human cancers

Ting, Chang Yong (2008) Quantitative and comparative analysis of WT1 (Wilms tumour suppressor) gene expression in human cancers. [Final Year Project Report] (Unpublished)

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Abstract

Wilms' tumour suppressor gene (WT1) can behave both as a tumour suppressor gene or an oncogene depending upon the cell type in which it is expressed. It is located at chromosome 11 p 13 and has 10 exons of which generates a 3 kb mRNA. An appropriate evaluation of WT 1 expression in tumour cells is important at present, because WTl is now regarded as a molecular target of immunotherapy for various malignant tumours. AJthough WTl has been proven to be associated with many different types of human cancers by previous studies, its expression in certain types of cancers have not been properly defined. Hence, this study is intended to provide an appropriate evaluation of WTl expression Ileveis in nasopharyngeal, colorectal, bladder and oral carcinomas via the Reverse Transcription Polymerase Chain Reaction (RT-PCR) strategy. Total RNA was extracted from human normal and carcinoma samples. Optimization strategies for PCR amplifications were performed utilizing one WTl primer set and one GAPDH primer set. The WTl primer set did not provide good results for accurate evaluation of WTl expression levels in all the samples, even though the GAPDH primer set provided positive results to verify the good quality of samples used.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2008.
Uncontrolled Keywords: Wilms' tumour suppressor gene (WT1), total RNA from human normal tissues and carcinomas (nasopharyngeal, colorectal, bladder and oral), Reverse Transcription, Polymerase Chain Reaction (RT -PCR), unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 17 Sep 2019 08:11
Last Modified: 17 Sep 2019 08:11
URI: http://ir.unimas.my/id/eprint/26883

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