Isolation and characterization of heterocyclic hydrocarbon degrading bacteria from industrial waste water

Mohammad Rahmat, Bin Derise (2015) Isolation and characterization of heterocyclic hydrocarbon degrading bacteria from industrial waste water. [Final Year Project Report] (Unpublished)

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Industrial development is the major factors for environmental pollution by releasing various compounds especially heterocyclic compound to the environment. This study was conducted to isolate heterocyclic hydrocarbon degrading bacteria from industrial wastewater. The wastewater samples were collected from wastewater sites of KTS Timber Industries and Bhd MAYGAIN Metal Industries Sdn. Bhd. The presence of the heterocyclic hydrocarbons (carbazole-CAR, diebenzothiophene-DBT and dibenzofuran-DBF) degrading bacteria was confirmed via enrichment techniques that used Minimal Salts Medium (MSM) broth as enrichment medium supplemented with heterocyclic hydrocarbon. Isolation of bacteria was successfully done by growing the bacterial cultures from enrichment stages onto MSM agar that also containing respective heterocyclic hydrocarbons (CAR, DBT and DBF) as sole sources of carbon and energy. Five different bacteria strains that can degrade heterocyclic hydrocarbons were successfully isolated. Then, five isolated bacteria were designated as T151, T152, M153, M154 and M155. Bacteria T151 and M153 are the CAR degrading bacteria, T152 and M154 are DBT degrading bacteria while M155 is DBF degrading bacteria. Interestingly, all isolated bacteria can degrade other heterocyclic hydrocarbons include biphenyl. All isolated bacteria were appeared as a Gram-negative rod shaped bacteria and all motile except M153 and M154 nonmotile. Based on the comparison of Gram staining and biochemical test results with the previous studies, T151 and T152 were suspected as genus Pseudomonas while M154 and M155 were suspected as genus Thalassospira and M 153 was suspected as genus Achromobacter

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2015.
Uncontrolled Keywords: Heterocyclic hydrocarbon, carbazole, dibenzothiophene, dibenzofuran, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 17 Sep 2019 02:38
Last Modified: 21 Aug 2023 08:17

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