Phytochemical, Pharmacophore and Anti-Diabetes Analyses of Moringa oleifera Lam. Seed

Ardy Mursyid, Bin Romli (2019) Phytochemical, Pharmacophore and Anti-Diabetes Analyses of Moringa oleifera Lam. Seed. Masters thesis, Universiti Malaysia Sarawak (UNIMAS).

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Abstract

Moringa oleifera Lam. is a well-known plant for its nutritious values and also famous for its ability to cure several diseases. Besides that, M. oleifera Lam. is known for treating diabetes in traditional therapeutic treatment. Diabetes mellitus is a serious diseased in every country nowadays. There’s a lot of medication for handling the diabetes type 2 and the mechanisms of each drug prescribed are different. One of the mechanisms is by disrupting the digestive enzyme which is a-amylase and a-glucosidase. Both of this enzyme responsible for digesting the starch to a smaller form of saccharides or known as glucose. Discovery a new potent a-amylase inhibitor from natural product source is the best idea in order to minimized the side effect caused by established drug inhibitor. The objectives of this study to, extract and isolate bioactive compounds, to elucidate the structure of isolated compound, and to determine a new potent a-amylase inhibitor from M. oleifera Lam. seed constituent by using the in-silico and in-vitro studies. The methanolic extract were partitioned in solvent with different polarity which is hexane, ethyl acetate and acetone. The isolation was done using column chromatography technique and resulted the isolation of four compounds which is 9- octadecanoic acid (HEX-1), and ethyl-9-octadecanoate (HEX-2) from the hexane partition, 4-(b-D-glucopyranosyl-1®4-a-L-rhamnopyranosyloxy)-benzyl isothiocyanate (EA-1) from ethyl acetate partition and 4-(α-L-rhamnosyloxy) benzyl isothiocyanate (ACE-1) from the acetone partition. The in-silico studies of isolated compounds were conducted using two pharmacophore modelling method which ligand based and structured based by using computer software Ligandscout 4.1. Result of in-silico studies showed that compound EA1 and ACE-1 exerting a good a-amylase inhibitor while the other two isolated compounds did not interact with a-amylase. The biological evaluation was conducted in three different iv assay which is cytotoxicity, antioxidant and in-vitro a-amylase inhibition assay. Cytotoxicity assay showed that acetone and hexane extract are less toxic compared to methanol and ethyl acetate. The antioxidant activity and in-vitro a-amylase inhibition assay resulted that compound EA-1 showing higher inhibition in both assay with the IC50 valued 22.84 µL/mL and 64.86 µL/mL while compound ACE-1 showed both mild antioxidant activity and in-vitro a-amylase inhibition assay with the IC50 valued 48.38 µL/mL and 145.50 µL/mL respectively. The result of in-vitro and in-silico studies showed that compound EA-1 and ACE-1 inhibit properties a-amylase inhibitor. The study concluded that M. oleifera Lam. have showed a great potential in inhibiting a-amylase.

Item Type: E-Thesis (Masters)
Additional Information: Thesis (MSc.) - Universiti Malaysia Sarawak , 2019.
Uncontrolled Keywords: amylase, glucosidase, pharmacophore modelling, ligand, in-silico, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, Postgraduate, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
R Medicine > RM Therapeutics. Pharmacology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: ARDY MURSYID BIN ROMLI
Date Deposited: 09 Sep 2019 14:22
Last Modified: 10 Sep 2019 00:27
URI: http://ir.unimas.my/id/eprint/26783

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