Morphological and Molecular Characterization of Potential Harmful Diatom, Pseudo-nitzschia from Sarawak Water with Description of a New Morphtype

Afiqah, Bt Hamilton Hanifah (2017) Morphological and Molecular Characterization of Potential Harmful Diatom, Pseudo-nitzschia from Sarawak Water with Description of a New Morphtype. [Final Year Project Report] (Unpublished)

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Abstract

Pseudo-nazchia may hung a harmful potential to both human and other aquatic inhabitants. Despite having extensive research done o n Pseudo-nlfzchia, there are only a few research done on Pseudo-nilzchia m Sa rawak waters. Hence, the main objeclives of this study are to determine and understand the morphological as well as molecular characterizati on of Pseudo-l1ifzchia in Sarllwak waters together. The samples for this research we re collected at Satang Salak, Samauang, KnchIng and Miri, Sarawak usmg 20 ,.lin plankton net and Van-Dom wa ter sampl er fo r wa ter sample collechon. The samples were cultu red to obtain monoclonal cultures for morphology and molecular characterization. Internal transcnpt spacer (ITS) region was amplified and sequenced. P. sp. I was consisted of lanceolate va lve and long tapering to the apices which differed from P. ptlngens. Max imum Pa rsilllOny (MP) and Neigbour-Joining (NJ) trees were reconstructed. The phylogenet ic tnferred the distinct relationship between P. sp. 1 and P. pungens by fom1ing sister clade. The fin ding in thiS study was proven by the high species diversity in coastal o f Sarawak.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2017.
Uncontrolled Keywords: Batang Salak, ITS, rDNA genes, AmneSIC Shellfish Poisonrng (ASP), unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 04 Jul 2019 03:14
Last Modified: 04 Jul 2019 03:14
URI: http://ir.unimas.my/id/eprint/25532

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