Rapid Amplification of cDNA Ends of Differentially Expressed Genes from Trunking and Non-Trunking Sago Palm (Metroxylon Sagu Rottb.)

Zulaikha, Binti Pol Ong (2019) Rapid Amplification of cDNA Ends of Differentially Expressed Genes from Trunking and Non-Trunking Sago Palm (Metroxylon Sagu Rottb.). Masters thesis, Universiti Malaysia Sarawak (UNIMAS).

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Metroxylon sagu can be found in two forms known as trunking and non-trunking sago palm. Trunking sago palm is the plant that contains high amount of starch accumulated in the pith of its trunk whereas non-trunking sago palm has a stunted growth of the trunk. Non-trunking sago palm can lead to wastage of various resources especially production of starch. The failure in the formation of trunk for non-trunking sago palm might be related to specific genes which are over or under expressed. Research which have been done previously on differential expressed transcripts of trunking and non-trunking sago palm showed that there were several partial gene transcripts successfully distinguished between trunking and non trunking sago palm. Since the gene sequences that are related to the formation and the failure to form a trunk of sago palm are still not fully determined, five of these transcripts were selected in an attempt to obtain full length cDNA sequences. The selected candidate genes in this study were DET1, DET2, DET3, DET4, and DET5. The 5’ and 3’ RACE were done by using total RNA from young leaves of sago palm. The 3’ends for five candidate genes were successfully obtained. For the 5’ends however, only DET1 gene sequence was successfully amplified. Then, new sequences were constructed and analysed, and found that each DET1, DET2, DET3, DET4, and DET5 are highly matched with peptidyl prolyl cis trans isomerase, beta-glucosidase, dnaJ, S-adenosylmethionine synthase and ascorbate peroxidase. Based on the additional sequence obtained in this study, this information can be used in a developing database specifically for trunking and non-trunking sago palm and to study further on the roles of these candidate genes in sago palm.

Item Type: Thesis (Masters)
Additional Information: Thesis (MSc.) - Universiti Malaysia Sarawak, 2019.
Uncontrolled Keywords: Trunking, non-trunking, cDNA sequence, 5’-RACE, 3’-RACE, sago, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, Postgraduate, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Date Deposited: 28 May 2019 08:18
Last Modified: 10 Jun 2020 08:00
URI: http://ir.unimas.my/id/eprint/25237

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