Identification of Proteins from Selected Medicinal Plants with Potential Anticancer Properties

Nurhazlina, Binti Hamdan (2019) Identification of Proteins from Selected Medicinal Plants with Potential Anticancer Properties. Masters thesis, Universiti Malaysia Sarawak.

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Abstract

Cancer is one of the most feared diseases in the world and is the second leading cause of ath worldwide. With the progression of modern technology, there is an upsurge in the use of natural resources as a direct medicine for cancer treatment. Combining the focus towards the use of natural resource for cancer medicine and the developing interest on peptides as the anticancer agent has led to this research study. The aim of the study was to discover proteins possessing potential anticancer activity from locally used medicinal plants. Several common plants that traditionally known to have medicinal properties which are Orthosiphon aristatus, Gynura procumbens, Clinacanthus nutans, Piper sarmentosum, Piper betle and Senna alata were used as the source samples. The crude protein extracts for these plants were extracted overnight by using two buffers; Phosphate Buffered Saline (PBS) and HEPES buffer. Protein profiling and quantification were performed by using SDS-PAGE and Bradford assay respectively. Nasopharyngeal cell lines (NP-69; normal cell line and HK-1; cancer cell line) were used to test the crude protein extracts for anticancer activity, resultedin narrowing down the targeted plant to Orthosiphon aristatus and Piper sarmentosum. The two plants samples were fractionized using ammonium sulfate precipitation technique and the fractionized sample cell toxicity values were measured by using MTT assay. The active fractions from both plants sample were identified using LC-MS/MS spectrometry resulted on a list of identified proteins.

Item Type: Thesis (Masters)
Additional Information: Thesis (MSc.) - Universiti Malaysia Sarawak , 2019.
Uncontrolled Keywords: Anticancer activity, protein, protein fractionation, cancer cells, cell toxicity, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, Postgraduate, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: NURHAZLINA BINTI HAMDAN
Date Deposited: 28 May 2019 07:17
Last Modified: 10 Jun 2020 08:06
URI: http://ir.unimas.my/id/eprint/25225

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