DNA Analysis of Scallops from Sarawak Exclusive Economic Zone (EEZ)

Lee, Shin Wei (2017) DNA Analysis of Scallops from Sarawak Exclusive Economic Zone (EEZ). [Final Year Project Report] (Unpublished)

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Abstract

Scallop, Amusium pleuronecte belongs to Family Pectinidae, a type of marine bivalve found in sandy bottom. This species had been sampled from Sarawak Exclusive Economic Zone (EEZ) during EEZ Survey Cruise and described by previous researcher. However, its molecular data has not been documented yet. Therefore, this study aims to carry out the DNA analysis of A. pleuronectes sampled from Sarawak EEZ. The length of the 16S rRNA gene sequences obtained from A. pleuronectes was 504 bp. The deletion at position 6 and transition at position 226 in DNA sequences can be observed when compared with the sample collected from different region. The 16S rRNA gene can be utilised to detect the genus level of species but demonstrated 0 % of genetic diversity within the same species. The phylogenetic tree of Neighbor-Joining (NJ) and Maximum Parsimony (MP) showed monophyletic of Amusium pleuronectes with the bootstrap values 100 and 99, respectively.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2017.
Uncontrolled Keywords: Scallounimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, Amusium pleuronectes, EEZ, 16S rRNA, DNA analysis, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 09 May 2019 03:04
Last Modified: 09 May 2019 03:04
URI: http://ir.unimas.my/id/eprint/24779

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