Preliminary Phytochemical and Molecular studies of Two Hibiscus sabdariffa L. Cultivars

Chieng, Siew Kee (2014) Preliminary Phytochemical and Molecular studies of Two Hibiscus sabdariffa L. Cultivars. [Final Year Project Report] (Unpublished)

[img] PDF (Please get the password from Digital Collection Development Unit, ext: 082-583932 / 082-583914)
Preliminary phytochemical and molecular studies of two...(fulltext).pdf
Restricted to Registered users only

Download (4MB)

Abstract

Hibiscus sabdariffa L. or Roselle is an introduced species from Africa. Its calyx is used to make beverages, jam, jelly and gelatine. The objectives of this study are to compare two cultivars of Roselle in Malaysia; red Roselle and dark red Roselle, in terms of morphology study of both cultivars. Besides that, this study attempt to develop polymorphic markers for the two cultivars of Roselle to provide information for future molecular breeding of this species. Another objective of this study is to produce phytochemical marker for the two Roselle cultivars based on their flavonoids composition. Field observation showed that the two cultivars cannot be distinguished at vegetative stage. However, the two Roselle cultivars can be differentiated at reproductive stage through the bract pattern of their young flower bud, the petal colour, the colouration and pattern at the petal base (known as `eye'), and the colour and length of calyx. A PCR product had been generated using two directed amplification of minisatellite-region DNA (DAMD) primers, URP30F and URP38F. The DAMD primer has to be optimized again in the future to produce useful information on polymorphic molecular markers of Roselle cultivars. Preliminary phytochemical analysis on leaf and calyx had successfully identified four tentative flavonoid classes i. e. isoflavones, flavonols, anthocyanidins and carotenoids, among a total of 11 spots found in leaf and calyx. Two flavonoid markers (Spot 5 and 6) were identified from the leaf of red Roselle cultivar; however their tentative flavonoid classes to date were unidentified. All of the data obtained provide evidences to assist in the identification of the two cultivars.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2014.
Uncontrolled Keywords: Hibiscus sabdariffa L., cultivars, leaves, calyces, morphology, directed amplification of minisatellite-region DNA, flavonoids, flavonoid markers,unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak.
Subjects: S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 16 Apr 2019 00:58
Last Modified: 16 Apr 2019 00:58
URI: http://ir.unimas.my/id/eprint/24495

Actions (For repository members only: login required)

View Item View Item