Establishment of axenic culture and callus induction of Dieffenbachia Seguine (JACQ.) Schott by Using Leaf Explants

Majidah, Binti Jamali. (2016) Establishment of axenic culture and callus induction of Dieffenbachia Seguine (JACQ.) Schott by Using Leaf Explants. [Final Year Project Report] (Unpublished)

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The genus Dieffenbachia consists of about 30 species of erect herbs native to tropical America. This plant is an ornamental monocotyledonous herbaceous plants prized for their attractive variegated foliage and tolerance of interior environments. Currently, tissue culture was seen as a method whereby Dieffenbachia stock could be free from systematic viral and bacterial pathogens. The current research emphasizes on the axenic culture establishment and callus induction of Dieffenbachia seguine (Jacq.) Schott by using leaf explants. The leaf explants were sterilized using 10% Clorox® solution (Sodium hypochlorite) with 5 minutes exposure time produced the highest percentage of axenic cultures which was 78.8%. The sterilized leaf explants were cultured onto Murashige & Skoog (MS) media supplemented with various concentrations of 2, 4-Dichlorophenoxyacetic Acid (2, 4-D) alone and Thidiazuron (TDZ). No callus was induced despite some of the leaf explants remained viable after certain period of time and most of the leaf explants results in browning condition also infested by fungus and bacteria. The poor callus induction from leaves was highly due to the presence of high poly-phenolic compounds within the Dieffenbachia plants. Future work should utilize different PGR‟s at different concentrations and also the use of different explants.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2016.
Uncontrolled Keywords: Dieffenbachia sp., ornamental plant, leaf explants, callus, Plant Growth Regulators (PGR), unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, , research, Universiti Malaysia Sarawak.
Subjects: S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 19 Mar 2019 01:28
Last Modified: 18 Aug 2023 08:03

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