Distribution of chloramphenicol-resistant gene in escherichia coli isolated from aquacultural environmental water

Cheng, Wei Kang. (2015) Distribution of chloramphenicol-resistant gene in escherichia coli isolated from aquacultural environmental water. [E-LPTA] (Unpublished)

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Abstract

Sarawak which is the largest state in Malaysia that contains largc space for aquaculture purposes. Antibiotic chloramphenicol is frequently used to quickly exterminate bacteria in food producing animal. The introduction of chloramphenicol has induced the formation of chloramphenicol-resistant bacteria including the Escherichia coli. These chloramphenicol-rcsistant genes can be found in E. coli in rivers near aquaculture farm. The objective of this research i to determine the distribution of chloramphenicol-resistant gene in E. coli isolated from aquacultural environment and environmental water. In this project 10 isolated bacteria from water samples were analyzed for chloramphcnicol antibiotic resistant genes (cat I, cat II, cat III and cat IV genes) by using mUltiplex PCR. All E. coli i olates are found to contain two different cat genes. All 10 isolates are found to have cat II genes. 5 isolates have cat IV genes, 3 isolates have cat IV genes and another 2 have cat I genes. This study can help in studying the significance in antibiotic resistant gene and how such differences contribute to the survival of the bacteria. The result from this research can also help inform the farmers to the risk of using chloramphenicol as antibiotic. The usage of chloramphenicol can cause the proliferation of chloramphenicol resistant bacteria and may spread to environmental water through water disposal from aquaculture farm.

Item Type: E-LPTA
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2015.
Uncontrolled Keywords: Aquaculture and environmental water samples, Cat genes, E. coli, Multiplex PCR, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, , research, Universiti Malaysia Sarawak.
Subjects: S Agriculture > S Agriculture (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 18 Mar 2019 08:48
Last Modified: 19 Mar 2019 00:26
URI: http://ir.unimas.my/id/eprint/24101

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