Eshtablishment of Contamination-free Culture and Induction of Organogenesis in Kapur Bukit (Dryobalanops beccarii Dyer)

Ang, Yong Kein (2009) Eshtablishment of Contamination-free Culture and Induction of Organogenesis in Kapur Bukit (Dryobalanops beccarii Dyer). [Final Year Project Report] (Unpublished)

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Kapur Bukit (Dryobalanops beccarii Dyer) a medium hardwood in Dipterocarpaceae has been identified as one of the timber species suitable for forest plantation in Sarawak. Establishment of planted forest requires a large amount of planting stocks and clonal materials are preferred. Conventional vegetative propagation of this species has not been very successful. The in vitro technique thus has been considered as an alternative for mass production of clonal material. As the first step towards development of a micropropagation protocol for D. beccarii, the first objective of this project is to establish contamination-free and viable explants in culture. The second objective is to initiate plant regeneration in vitro. Results of this study showed that immersing in 0.3% benomyl (w/v) for 15 minutes after surface sterilization and before transferring the explants from liquid to solid media could reduce fungal contamination to certain extent. Browning of culture which eventually killed the explants is currently a greater problem than microbial contamination in the establishment of axenic and viable culture. It was found that shoot-tip explants obtained from shoots emerged for some time did not turned brown as easily as those obtained from newly emerged shoots. Roots developed from callus induced by 2, 4-D on petiolule explants is reported for the first time for this species. Roots were observed to develop from the friable callus but not the compact callus. BAP and NAA at different concentrations have also been tested. To date, only callus formation was observed.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2009.
Uncontrolled Keywords: liquid culture, browning, organogenesis, petiolule explants, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak.
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 01 Mar 2019 00:44
Last Modified: 01 Mar 2023 03:57

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