Effects of substrate loading on sequential steps enzymatic hydrolysis of sago waste for sugar production

Zakaria, Bin Ismail. (2014) Effects of substrate loading on sequential steps enzymatic hydrolysis of sago waste for sugar production. [Final Year Project Report] (Unpublished)

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Abstract

Nowadays, the efficient utilization of agro-industrial by-products for conversion to a range of commercial value added bio-products, including bio-fuels, bio-chemicals, and bio-material is increases. Sago `hampas' contains mostly starch and lignocellulosic materials is a good choice to be used as a substrate in sequential step of enzymatic hydrolysis method to generate value added fermentable sugar. Therefore, this research project focused on the recovery of glucose from starch residue and cellulose of sago waste through the enzyme hydrolysis with different substrate loading such as 7 %, 10 % and 13 %. The commercial enzymes such as Liquozyme SC DS enzyme and Spirizyme Fuel enzyme were used to recover glucose from starch residue. Meanwhile, the Celluclast enzyme and ß-glucosidase enzyme were used to recover glucose from cellulose residue. In process to analyze and evaluate the concentration of glucose generated from starch and cellulose existed in sago `hampas', the filtered supernatant was tested with iodine test, analyzed by using dinitrosalicylic acid (DNS) method, `Glucose Analyzer' and `High Performance Liquid Chromatography (HPLC)'. The untreated and treated sago `hampas' was observed by using Scanning Electron Microscope (SEM) to show the present of starch granules in both samples. The 7 % of sago `hampas' was identified as an optimum and effective substrate loading to generate higher glucose compare with 10 % and 13 % of substrate loading whereby the percentage of total glucose conversion were 67.22 %, 66.01 % and 62.77 % respectively. Besides, through this research other fermentable sugars such as cellobiose and xylose were identified from sago `hampas' through the same hydrolysis method.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2014.
Uncontrolled Keywords: Sago `hampas', substrate loading, enzyme hydrolysis and fermentable sugar, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, , research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
S Agriculture > S Agriculture (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 01 Mar 2019 00:38
Last Modified: 01 Mar 2019 00:38
URI: http://ir.unimas.my/id/eprint/23732

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