Occurrence of Tetrodotoxin in Marine and Brackish Puffer Fish from East Malaysian Waters

Mohd Din, Monaliza and Mohamad, Samsur (2010) Occurrence of Tetrodotoxin in Marine and Brackish Puffer Fish from East Malaysian Waters. Monograph Aquatic Science Colloqium 2010.

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Abstract

Tetrodotoxin (TTX) is the toxic principle of puffer fish poisoning, a powerful and specific sodium channel blocker. TTX is exogenous in origin and accumulates in puffer fish through the food chain. Attempts were made to assess the toxicity, and verify toxin properties of marine and brackish puffers Lagocephalus lunaris, Tetraodon nigroviridis and Xenopterus naritus. The presence of TTX in puffer fish tissues were examined and verified by using mouse bioassay, Thin Layer Chromatography (TLC) and Gas Chromatography Mass Spectrometry (GC-MS). Toxicity assessment by mouse bioassay revealed that L. lunaris and T. nigroviridis was toxic (2.5 – 3.8 MU/g and 30.0 – 31.2 MU/g respectively). Meanwhile, thetoxicity scores of toxin extracted from tissues of X. naritus were 2.0 – 2.8 MU/g and the toxin were considered too mild to be detected since the mice did not die within 30 minutes after intra-peritoneally injection. Analysis of TLC showed that tissue extracts of L. lunaris, T. nigroviridis and X. naritus were positive for TTX. From the GC-MS analysis, it was unambiguously concluded that L. lunaris, T. nigroviridis and X. naritus exhibited TTX in their skin and muscle.

Item Type: E-Article
Additional Information: Universiti Malaysia Sarawak, UNIMAS
Subjects: A General Works > AC Collections. Series. Collected works
A General Works > AC Collections. Series. Collected works
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 06 May 2014 05:08
Last Modified: 06 May 2014 05:08
URI: http://ir.unimas.my/id/eprint/2318

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