Neuronal transcriptomic responses to Japanese encephalitis virus infection with a special focus on chemokine CXCL11 and pattern recognition receptors RIG-1 and MDA5

Shu, Pin Yu and Kien, Chai Ong and Perera, David and Kum, Thong Wong (2018) Neuronal transcriptomic responses to Japanese encephalitis virus infection with a special focus on chemokine CXCL11 and pattern recognition receptors RIG-1 and MDA5. Virology, 527. pp. 107-115. ISSN 0042-6822

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Abstract

Japanese encephalitis virus (JEV) causes central nervous system neuronal injury and inflammation. A clear understanding of neuronal responses to JEV infection remains elusive. Using the Affymetrix array to investigate the transcriptome of infected SK-N-MC cells, 1316 and 2737 dysregulated genes (≥ 2/−2 fold change, P < 0.05) were found at 48 hours post-infection (hpi) and 60 hpi, respectively. The genes were mainly involved in anti-microbial responses, cell signalling, cellular function and maintenance, and cell death and survival. Among the most highly upregulated genes (≥ 10 folds, P < 0.05) were chemokines CCL5, CXCL11, IL8 and CXCL10. The upregulation and expression of CXCL11 were confirmed by qRT-PCR and immunofluorescence. Pathogen recognition receptors retinoic acid-inducible gene-1 (RIG-1) and melanoma differentiation-associated protein 5 (MDA5) were also upregulated. Our results strongly suggest that neuronal cells play a significant role in immunity against JEV. CXCL11, RIG-1 and MDA5 and other cytokines may be important in neuropathogenesis

Item Type: Article
Uncontrolled Keywords: Japanese encephalitis virus, Transcriptome RNA microarray, Proinflammatory mediators, Neuronal infection, CXCL11, RIG-1, MDA5, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Divisions: Academic Faculties, Institutes and Centres > Institute of Health and Community Medicine
Depositing User: Ramji
Date Deposited: 20 Dec 2018 04:15
Last Modified: 25 May 2021 12:18
URI: http://ir.unimas.my/id/eprint/22847

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