Growth Kinetics of Ethidium Bromide Mutagenized Lipomyces starkeyi Strains

Micky, Anak Vincent and Lesley, Maurice Bilung and Latifah, Binti Suali and Afizul Safwan, Bin Azahari and Patricia Rowena, Anak Mark Baran and Elexson, Anak Nillian (2018) Growth Kinetics of Ethidium Bromide Mutagenized Lipomyces starkeyi Strains. Journal of Advanced Research in Applied Sciences and Engineering Technology, 11 (1). pp. 47-54. ISSN 2462-1943

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Abstract

Yeast and growth and biomass production are greatly influenced by the length of the incubation period during cultivation. Therefore, this study was conducted to investigate the growth kinetics of five Lipomyces starkeyi strains as determined by biomass production. The five L. starkeyi strains, namely L. starkeyi ATCC 12659, L. starkeyi MV-1, L. starkeyi MV-4, L. starkeyi MV-5 and L. starkeyi MV-8, were inoculated in sterilized Yeast Malt broth, and, incubated for 192 hr at ambient temperature. Biomass yields were assessed and calculated gravimetrically every 24 hr. Results indicated that the optimal biomass production of L. starkeyi ATCC 12659, L. starkeyi MV-1, L. starkeyi MV-4, L. starkeyi MV-5 and L. starkeyi MV-8 were at 120, 168, 144, 168 and 120 hr, with the concentrations of 6.64, 6.43, 9.78, 11.23 and 8.56 g/L, respectively. These results indicate that each L. starkeyi strain requires specific incubation period for the optimum production of fungal biomass. Therefore, by cultivating each L. starkeyi strain at the predetermined incubation period, biomass yields could significantly be improved for further downstream applications such as single cell protein and lipid production.

Item Type: Article
Uncontrolled Keywords: Lipomyces starkeyi, random mutagenesis, oleaginous yeasts, growth kinetics, fungal biomass, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 26 Sep 2018 03:02
Last Modified: 26 Sep 2018 03:02
URI: http://ir.unimas.my/id/eprint/21718

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