Characterization of enterobacteriaceae in Saremas Plantation, Sarawak

Hor, Mun Fong (2014) Characterization of enterobacteriaceae in Saremas Plantation, Sarawak. [Final Year Project Report] (Unpublished)

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This study was carried out to isolate, identify and characterize Enterobacteriaceae specifically from water samples in Saremas Plantation. Bacterial samples collected from Saremas Plantation were kept at a suitable condition until the bacteria are being brought to lab and process. The isolates were spread on selective nutrient agar and were screened for gram-negative, glucose-positive and oxidase-negative reaction. Forty four isolates were gram-negative and l isolate was gram positive. Twenty four isolates were coccus shape and 17 isolates were rod shape. Enterobacteriaceae is a family of rod shape, gram negative bacteria. Twenty five isolates that did not showed these Enterobacteriaceae characteristics were discarded. Seventeen isolates undergo different biochemical test such as methyl red test, indole test, Voges-Proskauer, citrate, motility, hydrogen sulphide gas production and urea utilization to differentiate the species. The bacteria identified and the results were compared to Bergey's Manual of Systematic Bacteriology. There are six genera identified from the family of Enterobacteriaceae. Pathogenic members of Enterobacteriaceae were identified such Yersinia, Morganella, Erwinia, Serratia and Citrobacter. Majority of the genus isolated was Yersinia with 35.29%. This is a concern as Saremas Plantation water flows could contaminate the water source of plantation and the housing areas of the plantation workers. The pathogenic strains found in the water samples can cause diseases to the animals and residents living nearby.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2014.
Uncontrolled Keywords: Enterobacteriaceae, Saremas Plantation, biochemical test, Bergey's Manual of Systematic Bacteriology, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 13 Aug 2018 02:36
Last Modified: 18 Oct 2023 08:47

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