Transformation of mungbeans via stratification of seeds

Nurul Liana, Binti Suardy (2015) Transformation of mungbeans via stratification of seeds. [Final Year Project Report] (Unpublished)

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Abstract

Mungbean (Vigna radiata (L.) Wilzeck) is one of the important crops used in daily life. Mungbean is rich in protein and easy to digest. Plant transformation can be conducted in several ways; either through direct DNA introduction or natural transfer mechanism. In this study, transformation was conducted using natural transfer mechanism via use of Agrobacterium tumefaciens. The Agrobacterium carrying the plamid pGSA1131, contains the antibiotic resistance towards chloramphenicol and a reporter gene, β-glucuronidase (GUS). For this work, the transformation was done via the use of stratified and non-stratified seeds. To facilitate the transformation, the seeds were exposed to vacuum infiltration and also the addition of acetosyringone. Analysis of putative transformants was done via extraction of plant DNA and detection of transgenes via polymerase chain reaction (PCR) technique. The PCR detecting the GUS reporter gene were undertaken and showed the presence of the gene in the putative transformants. It was also shown that stratification of the seeds facilitated the transfer and integration of transgenes.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2015.
Uncontrolled Keywords: Mungbean, Agrobacterium, pGSA1131, vacuum infiltration, acetosyringone, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 18 Jul 2018 04:16
Last Modified: 18 Jul 2018 04:16
URI: http://ir.unimas.my/id/eprint/20841

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