Effects of sodium bicarbonate in static culture of scenedesmus dimorphus

Sandra Lisa, Anak Gumpil. (2014) Effects of sodium bicarbonate in static culture of scenedesmus dimorphus. [Final Year Project Report] (Unpublished)

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Abstract

In this research, the chlorophyta Scenedesmus dimorphus was cultivated in different types of filtered water media, specifically tap water and seawater. The growth in term of biomass and lipid content of Scenedesmus dimorphus in filtered tap water and seawater were compared with the growth of Scenedesmus dimorphus cultured in modified tap water and seawater amended with of 20g/L, 25g/L and 30g/L of sodium bicarbonate, NaHCO3 respectively. The effects of different concentrations of NaHCO3 in modified tap water and modified seawater towards the biomass and lipid production were investigated. The cultivation of Scenedesmus dimorphus was done in static culture for 21 days. The dry cell weights analysis of the culture were conducted every 3 days and the cells were harvested on day 21 for lipid extraction to determine the lipid content. The result indicated that the cultivation of Scenedesmus dimorphus in filtered seawater yield the highest dry cell weight on day 18 of cultivation with 2.4g/L. The Scenedesmus cultured in filtered tap water recorded the highest lipid content with 31% of lipid recovery. Cultivation of Scenedesmus in modified tap water amended with 20g/L NaHCO3 showed the highest biomass yield with 1.31g compared to the other medium; however the lipid content was only 2.75%. Moreover, the growth of Scenedesmus dimorphus culture in modified seawater was stunted due to the abundant presence of white precipitate. The amount of NaHCO3 added into tap water and seawater should be taken into account when cultivating Scenedesmus dimorphus, so does the other factors such as light intensity, aeration and agitation to improve the growth of Scenedesmus in modified water.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2014.
Uncontrolled Keywords: Scenedesmus dimorphus, static culture, sodium bicarbonate, biomass, lipid, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak.
Subjects: S Agriculture > S Agriculture (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 18 Jul 2018 03:01
Last Modified: 18 Jul 2018 03:01
URI: http://ir.unimas.my/id/eprint/20812

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