Characterization of enterobacteriaceae from wild animals and water in conservation area in Saremas plantation

Chai, Shin Nei (2015) Characterization of enterobacteriaceae from wild animals and water in conservation area in Saremas plantation. [Final Year Project Report] (Unpublished)

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Abstract

The Enterobacteriaceae are gram-negative straight rod microorganism with capabilities of causing certain diseases, such as urinary tract infections (UTIs), diarrhoea and often fatal complication will occur if the Enterobacteriaceae penetrate into bloodstream and spread throughout whole body. Thus, this creates a critical public health concern. In this study, Enterobacteriaceae from wild animals and water in conservation area in Saremas Plantation, Miri, Sarawak were characterized using conventional biochemical tests, API 20E System and fingerprinting. Series of conventional biochemical tests were conducted, including Gram staining, Sulfide Indole Motility (SIM) medium, Kligler Iron Agar (KIA), Triple Sugar Iron (TSI) agar and oxidase test. Bacteria were grouped into two major clusters and various sub-clusters through (GTG)5—PCR analysis and dendrogram. There was a chance for bacteria distributed from wild animals into water and causes public health hazard to community within conservation area in Saremas Plantation. API 20E system revealed thirteen (54.2%) isolates with good identification level and above. The identification of the bacterial isolates using API 20E system via APIwebTM revealed seven bacterial species, including Enterobacter cloacae, Serratia marcescens, Serratia rubidaea, Citrobacter freundii, Rahnella aquatilis, Pantoea spp. I and Pantoea spp. 4.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2015.
Uncontrolled Keywords: Enterobacteriaceae, API 20E system, (GTG)s-PCR fingerprinting, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, , research, Universiti Malaysia Sarawak.
Subjects: Q Science > Q Science (General)
S Agriculture > SB Plant culture
S Agriculture > SF Animal culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Gani
Date Deposited: 17 Jul 2018 00:50
Last Modified: 17 Jul 2018 00:56
URI: http://ir.unimas.my/id/eprint/20756

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