Comparison of RPS15 expression levels between normal and tumour cells of human nasopharyngeal epithelium

Ong, Lian Ween. (2016) Comparison of RPS15 expression levels between normal and tumour cells of human nasopharyngeal epithelium. [Final Year Project Report] (Unpublished)

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Abstract

Ribosomal protein S 15 (RPS15) gene is known as protein-coding gene. RPS15 encodes for the ribosomal protein which is a constituent of small ribosomal subunit, 40S of ribosome. Recent studies have shown that RPS15 interact with Mdm2 which inhibits the degradation ofp53, a tumour suppressor. Furthermore, mutation in RPS15 gene have been reported to be involved in Dark-Blackfan Anaemia (DBA). This is evidence ofRPS15 gene involvement in clinical diseases. The clinical cases that drawn our interest in this study is nasopharyngeal carcinoma (NPC). A study has mentioned that various RPs are involved in NPC. But, the information about association of RPS15 in NPC is still limited. Therefore, in this study, we examine the expression level of RPS15 gene in normal and tumour nasopharyngeal epithelium. The cell lines that were used in this research are NP69 and HKI which are normal and tumour cell lines of nasopharyngeal respectively. In this study, the expression levels of RPS15 gene in both cell lines were analyzed using reverse transcriptase polymerase chain reaction (RT-PCR). RPS15 gene was found to be overexpressed in HKI compared to NP69 as the fold difference recorded to be greater than I. Moreover, statistical analysis using independent Student t test showed that the differential expression of RPS15 is significant. The overexpression of RPS15 gene in nasopharyngeal tumour cell line suggested the involvement ofRPS15 in the development ofNPC as it was found to have biological function in causing cancer in recent study. The study ofgene expression level ofRPS15 is expected to contribute in the effort to identify cancer biomarkers in the future.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2016.
Uncontrolled Keywords: RPS15, NPC, gene expression level, housekeeping gene, RT-PCR, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Saman
Date Deposited: 23 Apr 2018 08:26
Last Modified: 23 Apr 2018 08:26
URI: http://ir.unimas.my/id/eprint/20189

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