Cryopreservation of solanum lasiocarpum dunal using vitrification technique

Paula Maccie, anak Peter (2003) Cryopreservation of solanum lasiocarpum dunal using vitrification technique. [Final Year Project Report] (Unpublished)

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Abstract

A study was conducted to evaluate the effectiveness of vitrification technique in cryopreserving of Solanum lasiocarpum seeds. The seeds were immersed in Plant Vitrification Solution 2 (PVS2) with 0.0, 0.2, 0.4, 0.6, 0.8 and 1.0M of sucrose concentrations for 0, 30, 60, 90 and 120 minutes. Initial germination and moisture content of the seeds used were 51 % and 37.9% respectively. Vitrification solution with sucrose concentration of 0.6M exposed for 30 minutes gave the highest viability of 70% with 26% germination and 35.53 % moisture content Viability of the seeds decreased slightly after vitrification and stored in liquid nitrogen. Regression analysis showed that S. lasiocarpum seeds can be stored for 10 months and 2 weeks and 4 days after vitrification and kept in liquid nitrogen.

Item Type: Final Year Project Report
Additional Information: Project report (B.Sc.) - Universiti Malaysia Sarawak , 2003.
Uncontrolled Keywords: Solanum lasiocarpum Dunal, vitrification, viability, germination and regression analysis, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Saman
Date Deposited: 23 Apr 2018 07:29
Last Modified: 23 Apr 2018 07:29
URI: http://ir.unimas.my/id/eprint/20184

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