Protein profiling of disease piper nigrum

Nur Ezzati, Binti Hamdin. (2014) Protein profiling of disease piper nigrum. [Final Year Project Report] (Unpublished)

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Abstract

Phytophthora capsid is the main cause of the foot rot disease affected the P. nigrum plant. As the disease can cause economic loss to the agriculture, this research was performed to analyse the existence of any distinct protein between healthy and the diseased plant of the P. nigrum from the roots sample. Two methods of protein extraction were used to identify the difference of protein yield and better quality with quantification of protein using two assays which were Bradford assays and BeA assays. The SDS-PAGE analysis had revealed two different unique proteins band of molecular weight ~25 kDa and ~11 kDa from the diseased samples. Metaproteome has the potential to discover the new proteins contributing to analysis of pathogenesis related proteins. At the end of the experiment, the proteins were successful extracted from the roots of the infected plant with new protein profile discovered showing difference between the diseased and non-diseased P. nigrum.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2014.
Uncontrolled Keywords: Piper nigrum, Phytophthora capsid, proteomic analysis, metaproteome, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QK Botany
S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Saman
Date Deposited: 04 Apr 2018 03:46
Last Modified: 04 Apr 2018 03:46
URI: http://ir.unimas.my/id/eprint/19998

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