Lipid production in lipomyces starkeyi LSR6

Siti Hajar, Binti Abu Bakar. (2015) Lipid production in lipomyces starkeyi LSR6. [E-LPTA] (Unpublished)

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Abstract

The depletion of fossil-based resources is due to the high demand of petroleum based fuels. Plant oil that is being used as biodiesel feedstocks are not suitable as it competes with human food production, causes deforestation and habitat destruction. Therefore, oleaginous yeast such as Lipomyces starkeyi is being chosen as it offers various advantages. However, wild type strain of L. starkeyi often face limited lipid production. The objective of this study was to observe the lipid production of L. starkeyi LSR6 cultured under 2.5% (w/v) of glucose medium. The LSR6 strain was cultured under optimal growth. In addition, the L. starkeyi ATCC 12659 was also co-cultured under the same condition on separate bottle as control. Phenol sulfuric acid test and lipid extraction was performed to determine sugar consumption and lipid production. The highest growth of L. starkeyi ATCC 12659 and L. starkeyi LSR6 were obtained at 144 hr at 9.80 g/l and 10.60 g/l while the highest lipid production were about 3.67 g/l for L. starkeyi ATCC 12659 and 3.93 g/l for L. starkeyi LSR6. From these results, the percentage of lipid content of L. starkeyi ATCC 12659 and L. starkeyi LSR6 were 37.4% and 37.1%, respectively.

Item Type: E-LPTA
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2015.
Uncontrolled Keywords: L. starkeyi LSR6, glucose, lipid production, sugar consumption, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Saman
Date Deposited: 21 Mar 2018 08:46
Last Modified: 21 Mar 2018 08:46
URI: http://ir.unimas.my/id/eprint/19904

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