PCR detection of shiga-like toxin producing Escherichia coli isolated from river water and waste water

Nor Fazila, Bte Sulaiman. (2005) PCR detection of shiga-like toxin producing Escherichia coli isolated from river water and waste water. [Final Year Project Report] (Unpublished)

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A total of 87 isolates of waterborne escherichia col! we re isolated from three ri vers located at KOla Samarahan (Kuap River, Jernang River and Melaban River) and waste water al Kuching area (Malang Landfill and Sarav.-ak General Hospital ). Thirty isolates among (hem was investigated for [he presence of Shiga-like toxin producing £. coli particul arly Leoli 0157:1--17. Identification of the bacterial spec ies were based on morphological and biochemical test. The presence of f; coli 0 157:H7 and ilS virulence properties, Shiga-like toxin, SIX I and Stx2 were determined by muhiplex po lymerase chain reaction (peR) using two pairs of primers that simultaneously amplified segment Slx l and SIx2 genes in a single reaction. In this study, no presence o f Six I and SIx2 genes was observed from any of the water samples which indicat~d that the water SOurces are free from these pathogenic £'cofi strain. Microbiological quality analysis of river water and waste water were also conducted and revealed the higher [Otal plate count and co liform count of waSle water in comparison with river water. Fifthteen isolates were further chosen from the previous 30 isolates for antimicrobial susceptibility test using ten types of antimicrobial agents which ",ere amphicilin. bacitracin, carbenicillin, gentamicin, erythromycin, methici llin, nalidixic acid, streptomycin, tetracycline and vancomycin. From the antimicrobial resistant profile observed from this study, it was shown that E.cofi isolates from river water exhibited higher resistant level compared to isolates isolated from v,'aste water. These results could provide a database that would aid in the epidemiological investigation of the study area in future

Item Type: Final Year Project Report
Additional Information: Project report (BSc.) - Universiti Malaysia Sarawak, 2005.
Uncontrolled Keywords: mulliplex pe R, STEC, Ecoli O IS7:H7, Slxl . S1X2,unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Saman
Date Deposited: 20 Mar 2018 03:07
Last Modified: 22 Jan 2024 04:53
URI: http://ir.unimas.my/id/eprint/19879

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