Isolation and restriction site characterization of the tumour suppressor gene, p53, from total RNA prostate tissue

Samsunnisha Begum, Sultan Suhaibudeen. (2004) Isolation and restriction site characterization of the tumour suppressor gene, p53, from total RNA prostate tissue. [Final Year Project Report] (Unpublished)

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Abstract

p53 is a tumour suppressor gene, located at the shol1 arm of choromosome 17 at position 17p 13. I. The ability of p53 gene to induce apoptosis helps to maintain the genetic integrity of the genome. p53 gene comprises five conserved domains, which would result in deticiency of n.lnction, when mutated. The present study was to isolate p53 cDNA by Reverse Transcription -Polymerase Chain Reaction (RT-PCR) and "estriction site characterization by using several types of restriction enzymes. Total RNA from normal prostate tissue (AMBION Cat: #7988) was used as template. Several trials and optimizations were made tor PCR, with two specific primers on p53 gene. However, the desired length of 1.322kb product f'ailed to be amp litied.

Item Type: Final Year Project Report
Uncontrolled Keywords: p53, tumour suppressor gene, total RNA normal prostate tissue, restriction enzymes, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QR Microbiology
R Medicine > R Medicine (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Saman
Date Deposited: 20 Mar 2018 02:00
Last Modified: 19 Aug 2021 16:15
URI: http://ir.unimas.my/id/eprint/19870

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