Microsatellite DNA polymorphism of Macaca fascicularis populations in Malaysia

Jeffrine Japning, Rovie-Ryan and Faisal Ali, Anwarali Khan and Mohd Tajuddin, Abdullah (2017) Microsatellite DNA polymorphism of Macaca fascicularis populations in Malaysia. Malayan Nature Journal, 69 (4). pp. 287-300. ISSN 0025-1291

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Official URL: http://www.mnj.my/index.php/mnj/article/view/409


A total of 386 long-tailed macaques (M. fascicularis) from 17 populations in Malaysia was analysed using eight polymorphic microsatellite loci on a microfluidic-based platform (Agilent DNA 1000 Chips on the Agilent 2100 bioanalyzer) to identify and evaluate the genetic polymorphism and population structure. The microfluidic-based chip platform provides simple, reliable and cost effective approach for microsatellite studies. Genetic diversity estimates showed that the Peninsular Malaysian populations are slightly more diverse than their Bornean conspecifics. Furthermore, weak population structuring among the peninsula populations suggest that they comprise of genetically contiguous populations. The Sarawak and Labuan populations were differentiated from the peninsula populations except for the Sabah population which is due to the low sample number from the latter. Interestingly, no matching genotype was found among all samples indicating that the loci set can be used for individual identification of M. fascicularis.

Item Type: Article
Uncontrolled Keywords: Long-tailed macaques, sex bias dispersal, population structure, short tandem repeats, cross-species amplifications, microfluidic-based chip, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak.
Subjects: Q Science > QL Zoology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Anwarali Khan
Date Deposited: 13 Feb 2018 06:59
Last Modified: 13 Feb 2018 06:59
URI: http://ir.unimas.my/id/eprint/19630

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