Synthesis of first strand cDNA from metoxylon sago flower tissue

Raihanah, binti Hamdan. (2005) Synthesis of first strand cDNA from metoxylon sago flower tissue. [Final Year Project Report] (Unpublished)

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Abstract

Sago is well known for its starch producing trunk which becomes a basic food source for the locals especially in Sabah and Sarawak. However, little is known about their molecular characteristic. So, this study will emphasize on the construction of cDNA library from flower tissue. With the generation of a cDNA library it is hoped that it could later assist in a comparative study since the gene activities are different in every cell types. The generation of a cDNA library would require abundant amount of mRNA molecules. This can be achieved by extracting total RNA from the inflorescence. The total RNA was successfully extracted and the first strand cDNA was synthesized with the presence of AMV reverse transcriptase enzyme. Alas, the cDNA library cannot be finished in time due to time restraint and in short supply of reagents.

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