Comparison of chikungunya virus culture propagated in two cell line; vero and C6/36 cell lines

Ridzwanurahim, bin Nazimudin (2015) Comparison of chikungunya virus culture propagated in two cell line; vero and C6/36 cell lines. [E-LPTA] (Unpublished)

[img]
Preview
PDF
Comparison of chikungunya virus. (24 pages).pdf

Download (8MB) | Preview
[img] PDF (Please get the password from Digital Collection Development Unit, ext : 3932 / 3914)
Comparison of chikungunya virus. (fulltext).pdf

Download (16MB)

Abstract

Chikungunya is a mosquito-borne disease that has afflicted millions of people worldwide. Infection by the Chikungunya virus (CHIKV) causes symptoms including fever, rashes and arthralgia. The main aim of this study is to compare CHIKV propagated in two cell lines; Vero and C6/36 cell lines to identify the more conducive cell line for downstream assays. This was done by first culturing the cell lines before infecting the cell lines with the virus for viral replication. Cytopathic effects were observed in both cell lines, viral harvest titres were determined through viral plaque assay, and reverse-transcription polymerase chain reaction (RT-PCR) was conducted to confirm CHIKV along with viral RNA quantification. Findings suggest the C6/36 cell line to be the more conducive cell line.

Item Type: E-LPTA
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2015.
Uncontrolled Keywords: Chikungunya, vero cell line, c6/36 cell line, comparison, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QL Zoology
R Medicine > RB Pathology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Saman
Date Deposited: 30 Oct 2017 06:51
Last Modified: 30 Oct 2017 06:52
URI: http://ir.unimas.my/id/eprint/18373

Actions (For repository members only: login required)

View Item View Item