Screening, isolation and molecular characterization of @-amylase enzyme from basillus amyloliquefaciens umas 1002

Husna, Hj. Mohd Noor. (2003) Screening, isolation and molecular characterization of @-amylase enzyme from basillus amyloliquefaciens umas 1002. [Final Year Project Report] (Unpublished)

[img] PDF (Please get the password from TECHNICAL & DIGITIZATION MANAGEMENT UNIT, ext: 082-583913/ 082-583914)
Husna Mohd Noor.pdf
Restricted to Registered users only

Download (9MB)

Abstract

This study concerned about the screening, isolating and molecular characteristic of a-amylase gene of Bacillus amyloliquefaciens UMAS 1002. This cellulolytic and amylolytic bacterium was isolated from sago pith waste grew in minimal starch and carboxymethy1cellulose media. The production of a-amylase enzyme was undertaken by microbial degradation of the media that produced clear haloes around the colonies indicated amylolytic and cellulolytic activity. These colonies were selected for molecular analysis; genomic DNA extraction, restriction endonuclease digestion, Polymerase Chain Reaction (PCR), and genomic cloning. Since there are some problems, a gene encoding extracellular a-amylase enzyme could not be cloned and sequenced.

Item Type: Final Year Project Report
Additional Information: Project report (B.Sc.) - Universiti Malaysia Sarawak , 2003
Uncontrolled Keywords: Bacillus amyloliquefaciens, amylolytic, cellulolytic, a-amylase, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Saman
Date Deposited: 04 Oct 2017 03:34
Last Modified: 07 Jan 2022 03:32
URI: http://ir.unimas.my/id/eprint/17930

Actions (For repository members only: login required)

View Item View Item