Nucleotide polymorphism of sucrose synthase (susy) from kelampayan (neolamarckia cadamba)

Chong, Hui Nei. (2013) Nucleotide polymorphism of sucrose synthase (susy) from kelampayan (neolamarckia cadamba). [Final Year Project Report] (Unpublished)

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Abstract

Neolamarckia cadamba has high commercial value as its timber used as raw materials for production of plywood, pulp and paper, boxes and crates, light construction, dug-out canoes and furniture. Sucrose synthao;e (SuSy) catalyzes reversible formation of sucrose (Suc) and uridine diphosphate (UDP) into fructose (fru) and UDP-glucose (UDPG). UDPG is a major glycosyl donor for biosynthesis of cellulose, callose and starch. Single nucleotide polymorphism (SNP) is an important marker for selective breeding in the selection ofdesirable traits without growing plant until mature to observe phenotypic traits. The aim of this study was to identify the presence of SNP(s) in SuSy partial genomic sequence of Kelampayan. Targeted SuSy sequences were amplified by polymerase chain reaction (PCR). The -800bp of SIISy partial genomic sequences was subjected to BLASTn analysis to search for sequence homology in NCBl. Sequences were then aligned by using CLC Sequence Viewer 6 software to identify SNP(s). There were 37 SNPs and 2 InDel polymorphi ms detected in partial sequences of sllcrose synthase.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2013.
Uncontrolled Keywords: Neolamarckia cadamba, Polymerase Chain Reaction (PCR), Single Nucleotide Polymorphism (SNP), Sucrose Synthase (SuSy), UDP-glucose (UDPG), unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QK Botany
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Saman
Date Deposited: 21 Sep 2017 08:26
Last Modified: 21 Sep 2017 08:26
URI: http://ir.unimas.my/id/eprint/17746

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