Prevalence of vibrio cholerae and vibrio parahaemolyticus in wild birds and rodents from disturbed and pristine area

Chong, Joo Hui. (2009) Prevalence of vibrio cholerae and vibrio parahaemolyticus in wild birds and rodents from disturbed and pristine area. [Final Year Project Report] (Unpublished)

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Abstract

Vibrio cholerae and Vibrio parahaemolyticus are pathogens which cause gastroenteritis and other diseases to human. Wild birds and rodents in our environment play some role in transmitting these pathogenic bacteria to human. Hence, a study was carried out to determine the prevalence of V cholerae and V parahaemolyticus in wild birds and rodents from disturbed and pristine area. The birds and rodents from disturbed (Ta-ann Naman plantation in Sibu) and pristine habitat (Mt. Mulu) was captured and cloacal swab from each animal were collected. Isolation and identification was done on Thiosulphate Citrate Bile Salt agar and some biochemical test will be done do determine the bacteria species. Multiplex PCR was carried out to screen for the virulence genes of V cholerae which are tcp, ctx, zot and ace. As for V parahaemolyticus, the virulence gene are tdh and trh. The outcome is that the most of the samples collected from disturbed habitat were found to contain Vibrio spp. as compare to the samples collected from pristine habitat. Besides, not all the isolates of Vibrio cholerae and Vibrio parahaemolyticus contain virulence gene.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2009.
Uncontrolled Keywords: Vibrio cholerae, Vibrio parahaemolyticus, Multiplex PCR, pristine and disturbed habitat, birds and rodents,Vibrio cholerae, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QR Microbiology
Q Science > QR Microbiology > QR180 Immunology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Saman
Date Deposited: 21 Sep 2017 02:57
Last Modified: 12 Oct 2021 05:04
URI: http://ir.unimas.my/id/eprint/17710

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