Isolation and characterization of oil degrading bacteria from a local ecological sanitation (ECOSAN) system

Delima, anak Andrew Buntak. (2008) Isolation and characterization of oil degrading bacteria from a local ecological sanitation (ECOSAN) system. [E-LPTA] (Unpublished)

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Abstract

Eight bacterial isolates (B I, B2, B3, B4, B5, B6, B7 and B8) were isolated from the oil and grease trap of a Grey Water Treatment System (ECOSAN System) in Kenanga College. Four of the bacterial isolates were identified based on API 20E system as follows: B2 -Chromobacterium violeceum, B4 -Pseudomonas aeruginosa, B7 ,-Pseudomonas fluorescent/pulida and B8 -Klebsiella pneumonia pneumonia while three of them were identified based on 16S rRNA sequencing as follows: B3 -Bacillus sp. CNJ782 PL04, B5 -Pseudomonas aeruginosa Strain PDIOO and B6 -Pseudomonas aeruginosa strain RII. However, B 1 was unidentifiable. All the eight isolates were positively screened as oil degrading bacteria by using Rhodamine B agar. B4 can be concluded as having the highest degradation activity when incubated with both 1 % and 2% Tween 20 and Tween 80 compared to the other bacterial isolates.

Item Type: E-LPTA
Additional Information: Project Report (B.Sc.) - Universiti Malaysia Sarawak, 2008.
Uncontrolled Keywords: Bacterial isolates, oil degrading bacteria, degradation activity, grey water treatment, Bacterial genetics, Bacterial genetics Experiments, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Saman
Date Deposited: 21 Sep 2017 02:15
Last Modified: 21 Sep 2017 02:15
URI: http://ir.unimas.my/id/eprint/17704

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