Screening and cloning of amylase and cellulase genes from botryodiplodia theobromae

Elizah, Mohamed. (2004) Screening and cloning of amylase and cellulase genes from botryodiplodia theobromae. [Final Year Project Report] (Unpublished)

[img] PDF
Elizabeth Sinda - 24 pgs.pdf

Download (8MB)
[img] PDF (Please get the password from TECHNICAL & DIGITIZATION MANAGEMENT UNIT, ext: 082-583913/ 082-583914)
Elizabeth Sinda.pdf
Restricted to Registered users only

Download (14MB)

Abstract

This study concerned about the screening and cloning of amylase and ceHulase genes from Botryodiplodia theobromae. This fungus is found throughout the tropics and subtropics using numerous kinds of disease but especially rots of fruits and root crops during storage. In this study, B. theobromae used was isolated from mango and cultured on minimal media, minimal media containing starch and minimal media containing carboxymetylcellulose (CMC) as selective media. The existence of these enzymes was indicated by enzyme degradation of the media that produced clear haloes around the culture of fungus. Molecular analysis that have done on this fungus are: genomic DNA extraction, restriction endonuclease digestion. Polymerase Chain Reaction (PCR). cloning and a few confirmation test for clones. In this study. clones of amylase and cellulase and genes were success produced.

Item Type: Final Year Project Report
Additional Information: Project report (BSc.) - Universiti Malaysia Sarawak, 2004.
Uncontrolled Keywords: Botryodiplodia theobromae, amylase. cellulose, Fungi, genetic, reproductions, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > QK Botany
S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Saman
Date Deposited: 15 Sep 2017 07:32
Last Modified: 06 Jan 2022 07:07
URI: http://ir.unimas.my/id/eprint/17616

Actions (For repository members only: login required)

View Item View Item