Recombination between linear double-stranded DNA substrates in vivo

Narayanan, Kulathuramaiyer and Sim, Edmund U. H. and Ravin, Nikolai V. and Choon, Weng Lee (2009) Recombination between linear double-stranded DNA substrates in vivo. Analytical Biochemistry, 387 (1). pp. 139-141. ISSN 0003-2697

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Abstract

Recombineering technology in E. coli enables targeting of linear donor DNA to circular recipient DNA using short shared homology sequences. In this work, we demonstrate that recombineering is also able to support recombination between a pair of linear DNA substrates (linear/linear recombineering) in vivo in E. coli. Linear DNA up to 100 kb is accurately modified and remains intact without undergoing rearrangements after recombination. This system will be valuable for direct in vivo manipulation of large linear DNA including the N15 and PY54 prophages and linear animal viruses, and for assembly of linear constructs as artificial chromosome vectors.

Item Type: E-Article
Uncontrolled Keywords: BAC; linear; recombineering; recombination; phage; E. coli; chromosome; telomere, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak
Subjects: Q Science > QD Chemistry
R Medicine > R Medicine (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 18 Jul 2017 06:58
Last Modified: 18 Jul 2017 06:58
URI: http://ir.unimas.my/id/eprint/16918

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