Escherichia coli bactofection using Lipofectamine

Kumaran, Narayanan and Choon, Weng Lee and Aurelian, Radu and Edmund, Ui Hang Sim (2013) Escherichia coli bactofection using Lipofectamine. Analytical Biochemistry, 439 (2). pp. 142-144. ISSN 0003-2697

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Successful gene delivery into mammalian cells using bactofection requires entry of the bacterial vector via cell surface integrin receptors followed by release of plasmid DNA into the cellular environment. We show, for the first time, that addition of the DNA transfection reagent Lipofectamine improves entry of invasive Escherichia coli into HeLa cells and enhances up to 2.8-fold green fluorescent protein (GFP) expression from a reporter plasmid. The addition of Lipofectamine may be applicable to other bacterial vectors to increase their DNA delivery efficiency into mammalian cells.

Item Type: Article
Uncontrolled Keywords: Bactofection; DNA delivery; E. coli; Invasin; Lipofectamine; Transfection reagent, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak
Subjects: Q Science > QR Microbiology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Saman
Date Deposited: 18 May 2017 01:32
Last Modified: 18 May 2017 01:44

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