Adaptation of in vitro cytoadherence assay to Plasmodium knowlesi field isolates

Farrah, A Fatih and Siner, Angela and Atique M, Ahmed and Lu, Chan Woon and Craig, Alister G and Staines, Henry M and Balbir, Singh and Sanjeev, Krishna and Cox-Singh, J. (2010) Adaptation of in vitro cytoadherence assay to Plasmodium knowlesi field isolates. Malaria Journal, 9 (2). ISSN 14752875

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P. knowlesi was the first Plasmodium species in which antigenic variation was observed. Variation was due to schizont infected cell agglutination (SICAvar) antigens expressed by the parasite and transported to the exposed surface of the host erythrocyte [1]. PfEMP1 is P. falciparum’s orthologue of P. knowlesi’s SICA proteins [2]. In P. falciparum PfEMP1 is associated with infected erythrocytes binding to receptors such as ICAM-1 expressed on the endothelial cells of the host microvasculature. Here, we use a static protein assay [3] to determine if naturally occurring human P. knowlesi infections can cause erythrocytes to bind to ICAM-1, VCAM-1 and CD36.

Item Type: Article
Uncontrolled Keywords: P. knowlesi, Plasmodium, vitro cytoadherence assay, research, Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education
Subjects: Q Science > Q Science (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Medicine and Health Sciences
Depositing User: Karen Kornalius
Date Deposited: 04 Apr 2017 03:52
Last Modified: 04 Apr 2017 03:52

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