Comparative analysis of RPS15 expression between tumour cells of human colorectal system

Teh, Zy Ying (2016) Comparative analysis of RPS15 expression between tumour cells of human colorectal system. [Final Year Project Report] (Unpublished)

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Abstract

Ribosomal proteins (RPs) are components of ribosomes which shown different expression patterns in cancer cells. The changes of RP expression pattern are associated with development and progression of tumour cells. There is lack of exisiting data comparing the expression levels of RPS15 in cancer cell lines. The main aim ofour research is to detect and compare the expression patterns of RPS15 in two cancer colon cell lines. Gene expressions of RPS15 was studied in CRC line (SW480 and HVT116), with GADPH as a reference gene. Optimization of reverse transcriptase polymerase chain reaction (RT-PCR) was utilized and amplicaon produced was 115bp in length, before identifying the expression patterns of RPS15. RT-PCR was used to study expression levels of RPS15 and the runt throught agarose gel electrophoresis (AGE) to gain the result.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2016.
Uncontrolled Keywords: Ribosomes, Ribosomal protein, expression patterns, RPSI5, CRC, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: R Medicine > RB Pathology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Saman
Date Deposited: 17 Feb 2017 06:46
Last Modified: 17 Feb 2017 06:47
URI: http://ir.unimas.my/id/eprint/15354

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