Particle Image Classification in Digital Holographic Microscopy by Normalized Cross Correlation

Tamrin, K. F. and Rahmatullah, B. and Samuri, S. M. and Mahamud, S. T. (2016) Particle Image Classification in Digital Holographic Microscopy by Normalized Cross Correlation. In: 5th International Conference on Communications, Signal Processing Computing and Information Technologies (ICCSPCIT-2016), 16-17 December 2016, Hyderabad, India. (In Press)

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Abstract

Digital holographic microscopy is a promising technique for micro-scale fluid and solid measurements. It offers numerical advantage for digital image post-processing through manipulation of amplitude and phase embedded in the digital hologram. Previously, an off-axis digital holographic microscope was utilized to investigate aggregation of artificial platelets in the blood vessel. To mimic blood flow parameters to the minutest details, a cylindrical micro-channel was employed but this introduced astigmatism in the reconstructed particle images. This paper proposes the application of normalized cross correlation in feature matching technique so that astigmatic image can be efficiently classified prior to digital aberration correction. Here, automatic image classification relies on the computed normalized cross correlation coefficients with two dissimilar (astigmatic and focused) reference images. The method successfully classified eight images as astigmatic out of three hundred randomly chosen images. Limitation of the present method is also discussed. The method is foreseen useful for automatic image classification of a considerably large number of images usually acquired in digital holographic microscopy.

Item Type: Proceeding (Paper)
Additional Information: [1] R.D. Keane and R.J. Adrian, "Theory of cross-correlation analysis of PIV images," Applied scientific research, vol. 49, pp. 191-215, 1992. [2] J. Westerweel, G.E. Elsinga, and R.J. Adrian, "Particle image velocimetry for complex and turbulent flows," Annual Review of Fluid Mechanics, vol. 45, pp. 409-436, 2013. [3] Z. Liang, B. Yin, H. Liu, J. Mo, and S. Wang, "Displacement measurement of specimen surfaces with damaged areas by digital image correlation," Measurement, vol. 76, pp. 183-188, 2015. [4] B. Pan, K. Qian, H. Xie, and A. Asundi, "Two-dimensional digital image correlation for in-plane displacement and strain measurement: a review," Measurement science and technology, vol. 20, pp. 062001, 2009. [5] W.A. Simpson, G. Loffler, and L. Tucha, "Cross-correlation in face discrimination," Vision research, vol. 76, pp. 60-67, 2013. [6] P.A. Van den Elsen, E.-J. Pol, and M.A. Viergever, "Medical image matching-a review with classification," IEEE Engineering in Medicine and Biology Magazine, vol. 12, pp. 26-39, 1993. [7] K.F. Tamrin, B. Rahmatullah, and S.M. Samuri, "An experimental investigation of three-dimensional particle aggregation using digital holographic microscopy," Optics and Lasers in Engineering, vol. 68, pp. 93-103, 2015. [8] K.F. Tamrin, B. Rahmatullah, and S.M. Samuri. Measurement of signal-to-noise ratio (SNR) in off-axis particle holography using paraxial approximation. in The 2nd International Postgraduate Conference on Science and Mathematics. 2014. [9] B. Zitova and J. Flusser, "Image registration methods: a survey," Image and vision computing, vol. 21, pp. 977-1000, 2003. [10] J. Lewis. Fast normalized cross-correlation. in Vision interface. 1995. [11] S.A. Wormald and J. Coupland, "Particle image identification and correlation analysis in microscopic holographic particle image velocimetry," Applied Optics, vol. 48, pp. 6400-6407, 2009. [12] K.F. Tamrin, B. Rahmatullah, and S.M. Samuri. Development of an off-axis digital holographic microscope for large scale measurement in fluid mechanics. in 2nd International Multidisciplinary Microscopy and Microanalysis Congress, Oludeniz, Turkey. 2015. [13] K.F. Tamrin and B. Rahmatullah, "A review on noise suppression and aberration compensation in holographic particle image velocimetry," Cogent Physics, vol. 3, pp. 1142819, 2016. [14] K.F. Tamrin, B. Rahmatullah, and S.M. Samuri, "Aberration compensation of holographic particle images using digital holographic microscopy," Journal of Modern Optics, vol. 62, pp. 701-711, 2015. [15] K.F. Tamrin, B. Rahmatullah, and S.M. Samuri. Astigmatism compensation in digital holographic microscopy using complex-amplitude correlation. in 23rd Scientific Conference of the Microscopy Society Malaysia, Tronoh, Perak. 2014. [16] F. Zhao, Q. Huang, and W. Gao. Image matching by normalized cross-correlation. in 2006 IEEE International Conference on Acoustics Speech and Signal Processing Proceedings. 2006.
Uncontrolled Keywords: image classification, digital holographic microscopy, cross correlation, pattern matching, feature matching, Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education
Subjects: Q Science > QC Physics
T Technology > TK Electrical engineering. Electronics Nuclear engineering
Divisions: Academic Faculties, Institutes and Centres > Faculty of Engineering
Depositing User: Tamrin
Date Deposited: 04 Jan 2017 02:04
Last Modified: 24 Jan 2017 03:20
URI: http://ir.unimas.my/id/eprint/14769

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