Isolation and molecular analysis of humic acid-tolerant bacteria that are resistant to pesticides

Chia, Soo Yee (2004) Isolation and molecular analysis of humic acid-tolerant bacteria that are resistant to pesticides. [Final Year Project Report] (Unpublished)


Download (395kB) | Preview
[img] PDF (Please get the password from Digital Collection Development Unit, ext: 3932 / 3914)
Restricted to Registered users only

Download (393kB)


Twenty two bacterial strains isolated from peat soils in a pineapple plantation were analyzed. Fourteen of the Gram-negative strains were tentatively classified into 12 genera that included Thiobacillus, Acinetobacter, Acidiphilium, Enterobacter, and Pseudomonas. 91% of the isolates were able to tolerate 2,4-dimethylamine with concentrations of up to 10,000 ppm, while 60% of the isolates were resistant to malathion and chlorpyrifos with concentrations of up to 50,000 ppm. As for antibiotics, 82% of the isolates able to survive in carbenicillin agar with concentrations of 1000 ug/ml. 70% of the isolates can survive in both tetracycline and streptomycin media. Four bacterial isolates, C105 (Acidiphilium cryptum), C102 (Sulpholobus spp), C108 (Leptospirillum spp) and Y104 (Pseudomonas flouresc/putida) that were pesticide resistant were analyzed for the presence of plasmid. No plasmid was detected in antibiotic resistant isolates except for strains designated as Y 103 and C 105. The sizes of the isolated plasmids were estimated ranging from 6 kb to 54 kb. Further studies are required to investigate the genes which confer resistance properties in the acidophiles.

Item Type: Final Year Project Report
Additional Information: Project report (BSc.) - Universiti Malaysia Sarawak, 2004.
Uncontrolled Keywords: Acidophiles, Pesticide resistance, Antibiotic resistance, Plasmid, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: Q Science > Q Science (General)
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 24 Mar 2014 04:14
Last Modified: 15 Apr 2016 01:26

Actions (For repository members only: login required)

View Item View Item