Generation of expressed sequence tags (ESTs) database and isolation of alcohol dehydrogenase gene from young leaf samples of metroxylon sagu

Wee, Ching Ching. (2011) Generation of expressed sequence tags (ESTs) database and isolation of alcohol dehydrogenase gene from young leaf samples of metroxylon sagu. Masters thesis, Universiti Malaysia Sarawak, (UNIMAS).

[img] PDF
Ching Ching (24 pages).pdf

Download (1MB)
[img] PDF (Please get the password from Technical & Digitization Management Unit, ext: 082-583913/ 082-583914)
Ching Ching (fulltext).pdf
Restricted to Registered users only

Download (19MB)

Abstract

Alcoholic fermentattion in plants with reduction of acetaldehyde by alcohol dehydrogenase to produce energy is one of the metabolic pathways that enables plant to withstand stress. Sago plam is important to the state of Sarawak as one of the most important crops that bring huge revenue. It has the ability to withstand stress by growing well in waterlogged area. Shoots of sago palm in the early development stages has a high alcohol dehydrogenase gene expression. In this study, the gene expression in selected sago palm tissue was studied via the generation of expressed sequence tags of young leaves and isolation of full length adh cDNA from sago palm leaves using a combination of RACE and DNA walking method.

Item Type: Thesis (Masters)
Additional Information: Thesis (M.Sc.) -- Universiti Malaysia Sarawak, 2011.
Uncontrolled Keywords: Alcohol, Alcohol Denatured, Alcohol Biotechnology, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, Postgraduate, research, Universiti Malaysia Sarawak
Subjects: S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Saman
Date Deposited: 01 Nov 2016 02:53
Last Modified: 14 Oct 2021 13:40
URI: http://ir.unimas.my/id/eprint/14139

Actions (For repository members only: login required)

View Item View Item