Pseudo-nitzschia kodamae sp. nov. (Bacillariophyceae), a toxigenic species from the Strait of Malacca, Malaysia

Sing, Tung Teng and Hong, Chang Lim and Po, T.L and Viet, Ha Dao and Bates, Stephen S. and Chui, P.L (2014) Pseudo-nitzschia kodamae sp. nov. (Bacillariophyceae), a toxigenic species from the Strait of Malacca, Malaysia. Harmful Algae, 34. pp. 17-28. ISSN 1568-9883

[img]
Preview
PDF
Pseudo-nitzschia kodamae sp. nov. (Bacillariophyceae), a toxigenic (abstract).pdf

Download (190kB) | Preview
Official URL: http://www.sciencedirect.com/science/article/pii/S...

Abstract

A recent field survey of Pseudo-nitzschia species from coastal waters of Malaysia demonstrated the presence of a novel morphotype, P. sp. Port Dickson, in the Strait of Malacca. In this study, we revisited the site and established five clonal cultures of this morphotype, assessed the strains’ morphology and genetics, and delineated it as a novel species. As observed by electron microscopy, these strains showed morphological features identical to those of the previous field specimens designated as P. sp. Port Dickson. The cells differ from other Pseudo-nitzschia species in the P. pseudodelicatissima complex sensu lato by their lower densities of fibulae, striae and band striae in 10 mm. Molecular data of the nuclear encoded large subunit ribosomal rRNA gene and the internal transcript spacer region (ITS) further supported the delineation of this novel lineage. Based on both morphological and molecular data, P. sp. Port Dickson is considered to represent a new species, for which we propose the name Pseudo-nitzschia kodamae sp. nov. Production of domoic acid (DA) in the strains was examined by FMOC–LC–FLD. Only strains of P. kodamae were observed with a peak corresponding to DA, giving a concentration of 1.2– 42.5 pg DA cell�1. Screening of Pseudo-nitzschia caciantha, Pseudo-nitzschia batesiana, Pseudo-nitzschia fukuyoi and Pseudo-nitzschia lundholmiae cultures from the same waters, however, showed no detectable DA. This is the first report of a potentially toxigenic Pseudo-nitzschia species from the region.

Item Type: Article
Uncontrolled Keywords: Domoic acid, Malacca Strait, Pseudo-nitzschia, P. kodamae,unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 07 Sep 2016 23:22
Last Modified: 07 Sep 2016 23:22
URI: http://ir.unimas.my/id/eprint/13418

Actions (For repository members only: login required)

View Item View Item