Characterisation of Lipids from Litsea graciae and Litsea cubeba Fruits and Their Biological Activities

Jenny Sze Woon, Pui (2015) Characterisation of Lipids from Litsea graciae and Litsea cubeba Fruits and Their Biological Activities. [Final Year Project Report] (Unpublished)

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Abstract

Lipids extracted from the fruits of Litsea graciae and Litsea cubeba were selected for this study. The samples were extracted using Soxhlet extraction technique and analysed by Gas Chromatography-Mass Spectrometry, and chemical analysis such as acid value and saponification value. The average % (w/w) yield of lipid from L. graciae (2.18 %  2.06 % for fresh flesh; 3.12 %  0.31 % for fresh seed and 20.28 %  1.75 % for dried seed powder) and L. cubeba (6.17 %  1.17 %) was identified. Both lipids have an acid value of 29.05. L. cubeba gave saponification value of 108.46  3.24 while L. graciae gave 96.31  5.84 and 174.85  3.2 for the Soxhlet extracted flesh and dried seed powder, respectively. Palmitoleic acid was the most abundant unsaturated fatty acids present in the fresh flesh of L. graciae (26.55 %) and fresh seed of L. cubeba (20.35 %). For the saturated fatty acids composition of L. graciae, palmitic acid was mostly found in fresh flesh (15.20 %), lauric acid in both fresh seed (75.59 %) and dried seed powder (80.26 %). Stearic acid was the most composition in L. cubeba fresh seed (50.31 %). Biological activity such as brine shrimp lethality assay and antioxidant assay was carried out. Both L. graciae (LC50 = 639.04 ppm and 777.75 ppm for solvent soaking extraction and Soxhlet extraction respectively) and L. cubeba (LC50 = 779.71 ppm) oil showed to have a low toxicity as their LC50 (50 % mortality concentration) is over 500 ppm. EC50 (50 % effective concentration) values for L. graciae lipids (solvent soaking extracts = 3.09 μg/mL and Soxhlet extracts = 2.01 μg/mL), and Soxhlet extracted L. cubeba lipid (4.91 μg/mL) calculated were lower than that of ascorbic acid standard (11.12  3.47 μg/mL).

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2015.
Uncontrolled Keywords: unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak, Litsea spp.; lipid yield; brine shrimp lethality assay; antioxidant assay
Subjects: Q Science > QD Chemistry
S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 18 Mar 2016 07:41
Last Modified: 27 Jun 2021 04:35
URI: http://ir.unimas.my/id/eprint/10999

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