Characterization and Expression Study of Prel2a and Prel2b on early vetebrate development

Jonathan, Chan Chia Jui (2015) Characterization and Expression Study of Prel2a and Prel2b on early vetebrate development. [Final Year Project Report] (Unpublished)

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Abstract

Proline-rich EVHlligand 1 (Prell) and Proline-rich EVHI ligand 2 (PreI2) belong to Prel gene family. Both genes are homologous and participate in cellular integrin activation. Pre12 interacts with GTPase called Ras in GTP-bound state and is essential in regulating cell adhesion and migration. It has been identified to involve in mechanism assisting endocytosis of epidermal growth factor receptor (EGFR) that participates in development of arious diseases such as cancer. However, the comprehensive and mechanistic model of protein signaling involving cell migration is still unavailable. Hence, Prel2 is selected as candidate gene with zebrafish (Danio rerio) in this study to examine its function. This tudy was done partly to verify the occurrence ofPrel2 gene duplication as well as to fulfill our understanding on the importance of Prel2 during vertebrate development. The methodology involves colony peR, plasmid extraction, DNA sequencing, zebrafish cultivation, RNA probe preparation, in situ hybridization, embryo mounting and microscopy. Through these experiments, Prel2 genes were characterized and plasmid with the gene construct was isolated, purified, and ready for RNA probe synthesis for in situ hybridization of zebrafish.

Item Type: Final Year Project Report
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2015.
Uncontrolled Keywords: unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate,research, Universiti Malaysia Sarawak,Prel gene family, EVH, Danio rerio, endocytosis, gene duplication
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
G Geography. Anthropology. Recreation > GN Anthropology
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 07 Mar 2016 01:11
Last Modified: 08 Nov 2017 03:48
URI: http://ir.unimas.my/id/eprint/10772

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