Simultaneous co-saccharification and fermentation of sago hampas for bioethanol production

Micky, Vincent and Empina, Jabang and Norizawati, Muhamad Nur and Ennry, Esut and Leo, Bulin Unting and Dayang Salwani, Awang Adeni (2015) Simultaneous co-saccharification and fermentation of sago hampas for bioethanol production. Agricultural Engineering International: CIGR Journal, 17 (2). pp. 160-167. ISSN 1682-1130

PDF Micky.pdf Download (149kB)

Abstract

Abundance of lignocellulosic biomass provides a good solution to the demands of energy crops in producing biofuel like biodiesel and bioethanol. In this study, bioethanol was produced from sago hampas via the Simultaneous co-Saccharification and Fermentation (Sc-SF) process, at 2.5% and 5.0% (w/v) solid loadings. The processing step in Sc-SF is virtually similar to that of Simultaneous Saccharification and Fermentation (SSF). However, during Sc-SF, two enzymes, amylase and cellulose, were added for the co-saccharification of sago starch and fiber. In addition, Saccharomyces cerevisiae was used to ferment the sugars in the hydrolysates. The Sc-SF samples were analyzed for carbohydrate residues, ethanol and acetic acid using the dinitrosalicylic (DNS) acid assay and High Performance Liquid Chromatography (HPLC).Results showed that the Sc-SF of the sago hampas showed high efficiencies of hydrolysis and ethanol production within the first six hours of fermentation. Highest glucose production was at 37.86 g/l for the 5.0% sago hampas load and 17.47 g/l for 2.5% sago hampas load. The highest ethanol production was observed in the broth with 5.0% sago hampas, with a theoretical yield of 80.50%. Meanwhile, the highest bioethanol yield in the sample with 2.5% sago hampas was 73.19%. This study indicated that bioethanol production via Sc-SF from starch rich agricultural residues such as sago hampas is feasible.

Actions (For repository members only: login required)

View Item