Establishment of axenic culture and callus induction of synsepalum dulcificum daniel (miracle berry)

Tan, Hong Wee (2015) Establishment of axenic culture and callus induction of synsepalum dulcificum daniel (miracle berry). [E-LPTA] (Unpublished)

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Abstract

Synsepalum dulcificum is a slow growing shrub from family Sapotaceae. The fruits contain high medicinal values which aid in chronic diseases due to the presence of active component Miraculin, a taste modifying properties. The current research emphasizes on the axenic cultures establishment and callus induction of Synsepalum dulcificum from leaf and seed explants. The sterilized leaf explants were obtained from both 25% Clorox® solution (Sodium hypochlorite) which produced 91.7% axenic cultures and 25% CaOCI solution (Calcium hypochlorite) that produced 94.4% axenic cultures with 20 minutes exposure time upon addition of 1% Benomyl solution respectively. The sterilized leaf explants were cultured onto Murashige & Skoog (MS) media supplemented with various concentrations of 2,4- Dichlorophenoxyacetic Acid (2,4-D), and Picloram. No callus was induced despite some of the leaf explants remained viable after certain period of time and most of the leaf explants results in browning conditions whereas callus was induced from seeds supplemented with 2.0 mg/L of 2,4-D after 3 weeks of culturing. The poor callus induction from leaves was highly due to the presence of high poly-phenolic compounds within the miracle berry plants. Future work should utilize different PGR‟s at different concentrations and also the use of different explants.

Item Type: E-LPTA
Additional Information: Project Report (B.Sc.) -- Universiti Malaysia Sarawak, 2015.
Uncontrolled Keywords: Synsepalum dulcificum, Miraculin, Axenic Culture, Callus Culture, Plant Growth Regulators(PGR), unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, undergraduate, research, Universiti Malaysia Sarawak
Subjects: S Agriculture > SB Plant culture
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 11 Feb 2016 06:58
Last Modified: 11 Feb 2016 06:58
URI: http://ir.unimas.my/id/eprint/10514

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