A Non-Toxigenic But Morphologically And Phylogenetically Distinct New Species Of Pseudo-Nitzschia, P. Sabit Sp. Nov. Bacillariophyceae)

Sing, Tung Teng and Po, Teen Lim and Hong, Chang Lim and Maria, Rivera-Vilarelle and Sonia, Quijano-Scheggia and Yoshinobu, Takata and Michael A., Quilliam and Matthias, Wolf and Stephen S., Bates and Chui, Pin Leaw (2015) A Non-Toxigenic But Morphologically And Phylogenetically Distinct New Species Of Pseudo-Nitzschia, P. Sabit Sp. Nov. Bacillariophyceae). Journal of Phycology, 51 (4). pp. 706-725. ISSN 1529-8817

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A new species of Pseudo-nitzschia (Bacillariophyceae) is described from plankton samples collected from Port Dickson (Malacca Strait, Malaysia) and Manzanillo Bay (Colima, Mexico). The species possesses a distinctive falcate cell valve, from which they form sickle-like colonies in both environmental samples and cultured strains. Detailed observation of frustules under TEM revealed ultrastructure that closely resembles P. decipiens, yet the new species differs by the valve shape and greater ranges of striae and poroid densities. The species is readily distinguished from the curve-shaped P. subcurvata by the presence of a central interspace. The morphological distinction is further supported by phylogenetic discrimination. We sequenced and analyzed the nuclear ribosomal RNA genes in the LSU and the second internal transcribed spacer, including its secondary structure, to infer the phylogenetic relationship of the new species with its closest relatives. The results revealed a distinct lineage of the new species, forming a sister cluster with its related species, P. decipiens and P. galaxiae, but not with P. subcurvata. We examined the domoic acid (DA) production of five cultured strains from Malaysia by Liquid chromatography-mass spectrometry (LC-MS), but they showed no detectable DA. Here, we present the taxonomic description of the vegetative cells, document the sexual reproduction, and detail the molecular phylogenetics of Pseudo-nitzschia sabit sp. nov.

Item Type: Article
Uncontrolled Keywords: domoic acid; ITS2; Malaysia; Mexico; Pseudo-nitzschia sabit; ribosomal RNA genes; secondary structure; sexual reproduction, research, Universiti Malaysia Sarawak, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Academic Faculties, Institutes and Centres > Faculty of Resource Science and Technology
Faculties, Institutes, Centres > Faculty of Resource Science and Technology
Depositing User: Karen Kornalius
Date Deposited: 25 Jan 2016 03:27
Last Modified: 22 Jun 2021 14:19
URI: http://ir.unimas.my/id/eprint/10307

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