Expression of Recombinant E1 Glycoprotein of Chikungunya Virus in Baculovirus Expression System

Henry Sum, Magdline Sia and Andrew, Anna and Aren Maling, Milda (2015) Expression of Recombinant E1 Glycoprotein of Chikungunya Virus in Baculovirus Expression System. Journal of biochemistry, microbiology and biotechnology, 3 (1). pp. 26-29. ISSN 2289-5779

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Abstract

Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, the envelope E1 gene of CHIKV was cloned and expressed in a baculovirus system. The recombinant E1 protein with N-term 6-His residues protein was successfully expressed and purified as confirmed by SDS-PAGE and western blot analysis. The seroreactivity of the recombinant protein was evaluated in immunoassay for anti-CHIKV IgM and IgG antibodies. The recombinant antigen showed 69% sensitivity and 100% specificity for anti-CHIKV IgG by dot blot assay. Detection of anti-CHIKV IgM by dot assay showed 79% sensitivity and 100% specificity. No cross reactivity of the antigen was observed with anti-dengue virus serum samples. The results strongly support that the recombinant E1 protein has potential to be used as diagnostic antigen. The used of the antigen in a dot blot assay gives an advantage for laboratory detection without the need of any specialised equipment.

Item Type: Article
Uncontrolled Keywords: Chikungunya virus, E1 glycoprotein, baculovirus system, anti-CHIKV IgM, immunoassay, unimas, university, universiti, Borneo, Malaysia, Sarawak, Kuching, Samarahan, ipta, education, research, Universiti Malaysia Sarawak
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Q Science > QR Microbiology > QR180 Immunology
Q Science > QR Microbiology > QR355 Virology
R Medicine > R Medicine (General)
Divisions: Academic Faculties, Institutes and Centres > Institute of Health and Community Medicine
Depositing User: Ramji
Date Deposited: 03 Nov 2015 07:23
Last Modified: 03 Nov 2015 07:23
URI: http://ir.unimas.my/id/eprint/9288

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